Dear Stanley: Advice on Performing Dilutions

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Publish Date: November 30, 2017

Dear Stanley,

I recently purchased Epower™ from Microbiologics and need to dilute it to lower concentrations for my tests. Do you have any advice on how to perform dilutions?


Pam from Cleveland, Ohio

Dear Pam,

Thank you for purchasing Epower! I know firsthand how confusing dilutions can be. That’s why my fellow experts at Microbiologics and I created some tools to help you understand how dilutions work. These resources can also teach you how to make a dilution scheme for achieving the concentration you need.

1. Microbiologics Dilutions Guide

The first tool is a technical guide available on our website. The guide includes simple descriptions and illustrations that walk you through the basics of how to perform dilutions. You can find the guide here.

2. How to Perform Serial Dilutions in Microbiology Video

The second tool is a video featuring our Dr. Karla Fjeld. The video provides step-by-step instructions on how to perform dilutions.

Sometimes it’s easiest to learn by example. My colleagues recently helped a customer create the dilutions scheme below. This customer purchased an Epower strain at E3 concentration (1000-9999 CFU/pellet) and their target concentration was 10-100 CFU/0.1 ml.

Step 1: Start with an Epower E3 pellet (which contains 1000-9999 CFU).

Step 2: Add 1 pellet to 10 ml of pH 7.2 Phosphate buffer and follow the Epower hydration steps (Suspension A). The concentration of Suspension A is now at 100-999 CFU/1.0 ml (or 10-99 CFU/0.1ml)

Step 3: As stated above, use a 0.1 ml aliquot from Suspension A which will yield 10-99 CFU – Bingo!

I hope these tools help you design the best dilution scheme for your test methods. If you have questions about performing dilutions while using Microbiologics products, please reach out to our Technical Support team at +1.320.229.7045 or


Stanley Staphylococcus

About Stanley

Stanley Staphylococcus is a Master Micro-Technologist at Microbiologics, where he is responsible for helping customers understand why microorganisms behave the way they do. You could say he’s somewhat of a psychologist. Microbiologics has been lucky to have Stanley, a graduate of Gram-Positive Cocci University, as a member of their renowned Technical Support Team for over 20 years. Stanley says his favorite type of people are microbiologists and he enjoys traveling far and wide to meet them. Amazingly, Stanley has been on every continent – even Antarctica!

How to submit inquiries: There are two ways to get Stanley’s help. You can email your questions to or you can simply submit an inquiry from our Dear Stanley page. For urgent issues, please contact our Technical Support Team at or 1.320.229.7045.

Written by Microbiologics

Microbiologics is the foremost biological products and services provider, focused on protecting the health and safety of people around the world. For over 50 years, they have been partnering with healthcare and life science laboratories, manufacturers and suppliers across the globe to co-create and provide biological control materials, assay services and consulting for microbiology, molecular diagnostics and virology. Headquartered in Saint Cloud, Minnesota, the company has additional facilities in California, Kentucky and Michigan. Microbiologics has advanced industry standards for biological controls by adopting the most rigorous testing and quality standards including ISO 13485, ISO/IEC 17025, and ISO 17034. The company has an extensive distribution network spanning over 150 countries. For more information, visit

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  1. Cheryl Mellish

    Thank you for sharing the dilution process of bacteria. It was very clear and concise.

  2. Lois Skinner

    We would like to have a Stanley Staphylococcus to display in our laboratory. We currently have 5 microbes that we purchased from Giant Microbes and would love to have Stanley join them on our display shelf. How would we be able to get one?

    Kindest regards,
    Greer Commission of Public Works Water & Wastewater Laboratory

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