Methods for Growth Success: Aerobic Microorganisms

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Publish Date: December 8, 2015

mo 3When growing microorganisms, several conditions must be considered to ensure successful results including correct temperatures and pH, adequate moisture and sufficient nutrients (carbon and nitrogen).

Even experienced microbiologists can run into growth issues with finicky microorganisms. With this in mind, we have created a new series of blog posts that will highlight a variety of tried and true growth methods for creating an environment best suited for reproduction of various microorganism species.

Aerobic Microorganism Method:

Agar: The majority of aerobic microorganisms, without any strict nutritive requirements, will grow using Tryptic Soy Agar (Soybean Casein Digest Agar), nonselective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar

Temperature: 35°C

Atmosphere: Aerobic

Growth Time: 24 to 48 hours

The following microorganisms can grow using this simple culture method:

Achromobacter sp. Klebsiella sp.
Acinetobacter sp. Kocuria sp.
Aerococcus sp. Leclercia sp.
Alcaligenes sp. Listeria sp.
Aneurinibacillus sp. Lysinibacillus sp.
Aquaspirillum sp. Macrococcus sp.
Arthrobacter sp. Mannheimia sp.
Bacillus sp. Methylobacterium sp.
Brevibacillus sp. Microbacterium sp.
Brevundimonas sp. Micrococcus sp.
Brochothrix sp. Morganella sp.
Budvicia sp. Nocardia sp.
Burkholderia sp. Novosphingobium sp.
Cedecea sp. Ochrobactrum sp.
Cellulosimicrobium sp. Paenibacillus sp.
Chryseobacterium shigense Plesiomonas sp.
Citrobacter sp. Proteus sp.
Corynebacterium sp. Providencia sp.
Cronobacter sp. Pseudomonas sp.
Curtobacterium sp. Ralstonia sp.
Deinococcus sp. Raoultella sp.
Delftia sp. Salmonella sp.
Edwardsiella sp. Serratia sp.
Elizabethkingia sp. Shigella sp.
Enterobacter sp. Sphingobacterium sp.
Enterococcus sp. Sphingomonas sp.
Escherichia coli Staphylococcus sp.
Exiguobacterium sp. Stenotrophomonas sp.
Geobacillus sp. Virgibacillus sp.
Hafnia sp. Yersinia sp.

Some organisms have special temperature requirements. Specific details for each strain are listed on our Recommended Growth Requirements document on our website. Check back for detailed descriptions of all our growth methods which will be featured in future posts.

Written by Microbiologics

Microbiologics is the foremost biological products and services provider, focused on protecting the health and safety of people around the world. For over 50 years, they have been partnering with healthcare and life science laboratories, manufacturers and suppliers across the globe to co-create and provide biological control materials, assay services and consulting for microbiology, molecular diagnostics and virology. Headquartered in Saint Cloud, Minnesota, the company has additional facilities in California, Kentucky and Michigan. Microbiologics has advanced industry standards for biological controls by adopting the most rigorous testing and quality standards including ISO 13485, ISO/IEC 17025, and ISO 17034. The company has an extensive distribution network spanning over 150 countries. For more information, visit

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