GPT Quiz

Media should be inoculated with how many CFU?

20 CFU

50 CFU

<100 CFU

1000 CFU

Which organization(s) publish GPT guidelines? Select all that apply.

USP, Ph. Eur., and JP

USDA

FDA

EMA

The CFU value of the standardized inoculum can be determined by counting the number of colonies on the previously approved batch of agar.

True

False

Why should GPT be performed?

Determine the media manufacturer that best suits the lab’s needs

Determine shelf-life of media prepared in-house

Test the proficiency of lab technicians

Qualify purchased media and new batches of media prepared in-house

All of the above

Similar growth should be expected on selective and non-selective agar.

True

False

What is the maximum number of passages the culture can be from the reference culture?

1

2

4

5

Laboratories perform the Growth Promotion Test to determine if media is suitable for recovering small numbers of microorganisms.

True

False

When should the Growth Promotion Test (GPT) be performed on media?

When a new shipment is received

When a new lot number is received

When a new batch is prepared

All of the above

What is the acceptance criteria for non-selective agar?

20 - 80 CFU

50% – 200% of the standardized inoculum

70% of the standardized inoculum

Comparable growth to the previously approved batch of medium

What is the acceptance criteria for selective agar?

20 – 80 CFU

50% – 200% of the standardized inoculum

70% of the standardized inoculum

Comparable growth to the previously approved batch of medium

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