MOLECULAR
MOLECULAR MICROBIOLOGY
Read articles about molecular microbiology from Microbiologics’ experts and other field experts.
COVID-19 and Proficiency Testing
I think by now it is safe to say that we are truly living in unprecedented times with the current Coronavirus pandemic. The majority of us have never experienced anything even remotely similar to this in our lives before. If you are currently a front-line worker in...
SARS-C0V-2 Tests & Their Targets
By Marylou Gibson, Ph.D Adapted from a post that originally appeared on LinkedIn. Of the more than 55 RT-PCR Test kits or assays currently available under Emergency Use Authorizations (EUA) by the FDA, few target the same genes. Many more similar PCR based tests are...
Importance of Testing a Positive Control When Performing a Diagnostic Assay
Positive controls are critical for ensuring diagnostic accuracy. This post examines reasons why positive controls are necessary, even during emergency situations.
Clinical Case File Results: Persistent Dysuria
Photo Source: Lind, Lancet 1982 Last week we shared the Clinical Case File of a patient with persistent dysuria and asked our readers to determine the cause of the symptom. Here are the poll results: Adenovirus (4%, 7 Votes) Herpes simplex virus (9%, 21 Votes) Human...
Clinical Case File: Persistent Dysuria
Read the case study below and then use your microbiology expertise to determine which pathogen is causing the symptoms. The results will be shared on August 1, 2019. Good luck! Background: Determining which sexually transmitted infection (STI) is causing uncomfortable...
Tips for Choosing and Using Microorganisms for Education
In 2016, two biologists from Indiana University estimated that there are about a trillion species of bacteria on earth.1 With so many choices, how can a teacher select the best strains for her microbiology class? Below are a few tips for choosing and using...
Ask a CAP Inspector: How Frequently Should Molecular Targets Be Rotated?
Q: How frequently should I test targets for my molecular multiplex testing assays? A: This is a common question for inspectors, vendors, and laboratorians. Unfortunately, there are no cut-and-dried answers, but there are some best practices that you can apply in your...
Dear Stanley: Investigating False Positive Results
Dear Stanley, Lately, our lab has been experiencing PCR results that don’t fit with what is expected. We will see occasional positive results in a negative sample. I think the issue could be related to contamination in the lab, but I’m not sure. Do you have any tips...
Microbiology’s Wild West: Cannabis Lab Leaders Talk Testing Methods and Regulations
Cannabis has become a widely accepted form of medicine for patients across the United States. These patients, many of whom are immunocompromised, rely on testing laboratories to ensure the safety of the products they use. The lack of consistent and clear regulations...
Ask a CAP Inspector: When Should a Rapid Diagnostic System Be Recalibrated?
Q: When should I validate or recalibrate my rapid diagnostic system? A: With all the rules and regulations, it’s hard to know when a diagnostic system validation or recalibration is required. For most multiplex PCR testing instruments, the requirements are a bit...
