Clinical Case File: Enteric Pathogen

by | Clinical, Food, Molecular

Publish Date: January 31, 2019

Source: CDC PHIL and NIAID

The case of an ambiguous food-borne pathogen causing an enteric infection is the first in our new series of clinical case files. Read the case study below and then use your microbiology expertise to determine which pathogen is causing the illness. Good luck!


Lately, it seems like every time we pick up a newspaper, scroll through a news feed on our phones or watch the evening news, we learn about another food recall. Lettuce, cauliflower, beef, turkey and chicken have recently been in the limelight.  Everywhere we turn we see new cases of food contamination, leaving us feeling like a run-in with contaminated food is inevitable.

Case Study:

It’s the weekend and you are out with your family at a local restaurant. You order a house salad and a juicy burger and enjoy the rest of your night out. The next evening you are miserable and present yourself to the emergency room with nausea, vomiting, abdominal pain and bloody diarrhea. Prior to this incident, you have no history of inflammatory bowel disease, previous diarrhea episodes or recent contact with individuals with diarrhea. So, what could be the cause?

The emergency room staff collects a stool specimen and sends it to the laboratory for a stool culture. The microbiology work up determines the pathogen is gram-negative and it exhibits the following biochemical profile:

Hydrogen sulfide (TSI) – Positive

Urease – Negative

Indol – Negative

Methyl Red – Negative

Phenylalanie deaminase – Negative

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Read the results post to find out which strain caused the infection. See the results.

Read Next – Dear Stanley: Molecular QC Best Practices

Written by Joshua Pulido, MHA, MT(ASCP)

Joshua X. Pulido, MHA, MLS(ASCP), is the Business Development Manager at Microbiologics where he leads the business activities for the Virapur division. Josh has nearly 20 years of experience in clinical diagnostics and is a certified Medical Laboratory Scientist through the American Society of Clinical Pathology. Prior to joining Microbiologics, Josh worked in marketing and sales in the Clinical Diagnostics industry for 11 years, and previously had nearly nine years of experience working in clinical laboratories as a technologist and supervisor. He earned his bachelor’s degree in Clinical Laboratory Science from Weber State University and holds a Master of Health Administration from Weber State University.

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